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- 연제번호 : P-345 북마크
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제목 Sequential Activation of AMPA Receptors and Glial Cells in a Pain Model of HLD 소속 college of medicine, Yeungnam University, Department of Physical Medicine and Rehabilitation 1 저자 Dong Gyu Lee1†, Dong Gyu Kwak1*† Objective: To investigate the glial cell and AMPA receptor activity over time after surgery for disc herniation pain model.
Methods: In total, 83 Sprague-Dawley rats were randomly assigned to the following groups: Control (n = 16), sham-operated (n = 4), rats for pain behavior evaluation (N=3), and nucleus pulposus-exposed groups for AMPA receptors (n = 30) and glial cell (n = 30). The rats were tested for mechanical allodynia; immunohistochemical staining for AMPA receptors (GluA1 and GluA2) and glial cells (OX-42 and glial fibrillary acid protein [GFAP]) in the spinal dorsal horn was performed on postoperative days 3, 7, and 14.
Results: Mechanical withdrawal thresholds decreased after surgery, and this effect was maintained for up to 14 days. Immunohistochemical expression of GluA1 and GluA2 in the spinal dorsal horn increased quantitatively on postoperative days 3 and 7 (P < 0.05) and reached levels similar to that of the control on postoperative day 14. Moreover, immunohistochemical expression of OX-42 and GFAP showed similar changes in AMPA receptors after surgery. Although the activity of AMPA receptors and glial cells achieved normalcy, mechanical withdrawal threshold of the hind paw remained decreased even 38 days after surgery.
Conclusions: The rat model of lumbar disc herniation showed increased expression of AMPA receptor and glial cell activity in the spinal dorsal horn at 3 and 7 days after the operation, which deceased to control levels at 14 days. The AMPA receptors and glial cells activation showed similar patterns after disc herniation operation
File.1: Fig1.jpgHyperalgesia in a rat model of lumbar disc herniation, induced by implantation of the nucleus pulposus to the left L5 nerve root. Withdrawal threshold in the ipsilateral paw was noted following surgery at each timepoint. POD = postoperative day; NP = nucleus pulposus exposed rat.File.2: Fig2.jpgImmunoreactivity staining for GluA1 and GluA2 in the dorsal horn of the L5-level spinal cord at 3, 7, and 14 days after surgery, respectively. GluA1 and GluA2 are diffusely present in all parts of the dorsal horn, but are intensely stained in the superficial lamina of the dorsal horn. POD = postoperative day.File.3: Fig3.jpgSemi-quantitative analysis of GluA1 and GluA2 immunoreactivity in the dorsal horn of spinal cord at POD3, POD7, and POD14. Mean green value of GluA1 and GluA2 were statistically significant higher at POD3 and POD7, compared with control. Values are expressed as mean ± standard error. POD=postoperative day; *P<0.05 versus the control group.
